Sandrine VERDISSON*, ** et 1, F. BAILLIEUL* et J.-C. AUDRAN*
*Laboratoire de Biologie et Physiologie Végétales
**Laboratoire de Zoologie et des Sciences de l'Environnement (EA-2069)
UFR Sciences, Université de Reims Champagne Ardenne, B.P.1039, 51687 Reims-02 (France)
Abstract : Difference in grape sensitivity to Botrytis cinerea attacks between cultivars was explained by differences in the epidermic tissue of the fruit. Therefore, this work was conducted to create a grape periclinal chimera, whose fruits would combine the skin of a Botrytis tolerant cultivar with a pulp of an another cultivar admitted its good organoleptic quality and productivity. In a first time, graftings of two cultivars (Chardonnay and Pinot noir) were conducted in vitro on 5 different media supplemented with various plant growth regulators. Adventitious shoots were only observed on medium containing BAP and GA3 from a mixed callus structure after four weeks of darkness followed by a light/dark regime. In a second time, RAPD analysis, conducted on these plants, showed their chimerical characteristics.
Keywords : Botrytis, Vitis vinifera L., chimera, regeneration, RAPD